User guide

Standards and Band Matching
15-15
Yellow bands are bands that the software cannot accurately match. The
matching algorithm is deliberately conservative to avoid incorrect labeling, so
a number of yellow bands may appear on the image.
Your next step will be to identify the yellow bands as either new band types,
or existing band types that could not be automatched.
To summarize:
• Green bands are known band types, based on your identification.
• Red bands are bands that have been automatically matched with the
known band types.
• Yellow bands are bands that have not been matched and are unclassified.
Displaying Band Types and Modeling Lines
To display the band type numbers on the image, select Show Band Types
from the Match menu or toolbar.
Band type modeling lines reveal the path along the gel image that the
software uses to match bands of the same type in different lanes. These lines
are based on the positions of the known (green) bands and any standards you
may have defined.
To display the band type modeling lines, select Show Band Models from the
Match menu or toolbar. The currently assigned band types will appear, as will
the modeling lines connecting the band types.
15.2.a Editing the Results of Band Matching
After you have matched the bands in your gel image automatically, you can
manually match the remaining yellow bands using the match tools on the
Match menu and toolbar.
You can use these tools to manually change the band type of a band, create
new band types, unmatch bands, or identify bands as outliers from your band
set.