User guide

Quantity One User Guide
15-14
If you have defined standards in the gel, a pop-up box will warn you that the
regression model for calculating band values will be restricted to point-to-
point semi-log. If you selected a different regression model when defining
standards, it will be changed.
The first time you click on an experimental lane with the Match command, a
pop-up box will prompt you to specify the matching tolerance.
Fig. 15-11. Query box: Apply matching to the whole lane?
Note: Tolerance is the minimum spacing that the matching model expects to
find between unique bands. It is expressed as a percent of lane height.
You can enter a value between 0.2 and 10 percent. If your bands are very
close together, enter a tolerance of 2.5 percent or less.
After you select a tolerance, click on Yes to automatically match all the bands
in the image. (Click on No to match only the specific band you clicked on.)
When you click on Yes, the bands in the lane you selected will change to
green, indicating that they are known band types that have been identified by
you. A band type number will appear next to each band.
The automatic matching mechanism will attempt to match the bands in your
image to the known band types. Matched bands are labeled in red, with the
number of the band type appearing next to each band. These matched bands
are connected by modeling lines.