User guide

Standards and Band Matching
15-13
After you remove this file, a new, empty ONEDPREFS.DBS database will be
automatically created the next time you open an image. You can use this to
begin a new archive.
15.2 Band Matching
To compare the similarity of the lanes in your image (using the phylogenetic
tree, similarity matrix, etc.), you must first identify all the bands in the gel and
match identical bands in different lanes. The functions for doing this are
located on the Match menu and toolbar.
Note: If you have run standards on your gels, you should define them before
proceeding. Multiple lanes of standards will facilitate the band matching
program; however, they are not required.
Fig. 15-10. Matching tools.
Select Match from the menu or toolbar, then click on a representative
experimental lane in your gel image. This may be a lane that contains most or
all of the bands that you are interested in, and/or a lane in which the bands
are particularly well-resolved. Each band in this lane will be designated as a
different “band type.”