User guide

Bands
14-25
14.8 Irregularly Shaped Bands in Lanes
If the bands in your lanes are irregularly shaped, you can use the contour or
drawing features to define them. These functions give you more control over
defining your bands than either Detect Bands or Create Bands.
Note: These tools are similar to the Volume Contour Tool and the Volume
Freehand Tool on the Volume menu, except that they are lane-dependent.
If you want to quantify objects without defining lanes first, see Chapter
16, Volume Tools.
Contoured or hand-drawn bands are quantitated based on the signal
intensity of all the pixels within the band boundary, using the following
formula:
Quantity = Sum of (intensity of pixel x pixel size) for all the pixels in
the boundary.
The intensity of a pixel is multiplied by the area of the pixel. This is done for
all the pixels that are within the contour or drawn boundary. The area of the
pixel is determined by the scan resolution of the image.
The resulting values have units of intensity x mm
2
.
Fig. 14-15. A contoured/drawn band scanned at 100 x 100 microns (micrometers).
0.1 mm
Contoured/drawn band showing individual
pixels with different intensities.
One pixel enlarged to
show dimensions.