User guide

Quantity One User Guide
14-20
Note: Note that Normalized Rf is derived from Relative Front; however,
Normalized Rf is calculated only for bands that have been modeled using
standards or band sets, and can change based on the modeling.
• Molecular weight/Isoelectric Point/Base Pairs/other units—This value is
determined by the type of standards defined for the gel, the band’s
position in the lane, and any modeling performed on the gel (via band
matching or multiple lanes of standards) to compensate for gel distortion
or smiling.
• Peak density—The intensity value of a band’s peak.
• Average density—The total intensity of the rows of pixels used to
generate the profile of a band, divided by the number of rows.
• Trace qty—The quantity of a band as measured by the area under its
intensity profile curve. Units are intensity x mm.
• Gauss Peak Density—The intensity value of a band’s Gaussian peak
(after Gaussian modeling).
• Gaussian Trace Quantity—The quantity of a band as measured by the
area under its Gaussian-fitted profile.
• Contour qty—The quantity of a band that has been identified using the
Contour or Draw Band tools. It is the sum of the intensities of all the
pixels within the band boundary multiplied by the area of each pixel.
Units are intensity x mm
2
.
• Relative qty—The quantity of a particular band in a lane expressed as a
percentage of either (1) the total quantity of all the bands in the lane or (2)
the total intensity data in the lane. The calculation method (% of Lane/%
of Bands in Lane) is set in Application Preferences under Edit >
Preferences.
• Normalized qty—The trace quantity of a particular band expressed as a
percentage of the quantity of a selected band type that is present in the
same lane.
If the quantity of the band you have selected is known, you can enter the
quantity and units next to the Quantity/Units prompt.
If you want to calibrate that band against known quantities, you can do so by
clicking on the Calibration button. You will be asked to select the calibration