User guide

Lanes
13-9
When detecting bands, you should select a Lane Width that is slightly wider
than the actual lanes in your gel.
Note: You must eliminate background intensity from your lanes using the Lane
> Lane Background command (section 13.2) before detecting bands.
You can adjust the sampling width of all the lanes in your image from within
the Detect Bands dialog box (see section 14.2.a). See section 14.1 for a full
discussion of the effect of sampling width on band quantitation.
13.1.h Creating a Lane Profile
After you have defined a lane, you can create an intensity profile of that lane
using the Plot Lane function. A lane profile provides a quick visualization of
the intensity of your sample data, and is also useful for determining the
amount of background noise in your image.
Select Plot Lane from the Lane menu or toolbar, then click on a lane. A profile
window will pop up.
Fig. 13-9. Profile of a defined lane.
To profile another lane, simply click on the lane with the Plot Lane command
still assigned to the mouse.
Intensity peaks
Background noise