User guide

Quantity One User Guide
12-28
You can use view tools like Zoom Box and View Entire Image in the Preview
Window just as you can in the main image window, to focus on particular
regions of interest. You can also use the ARROW keys to move the image within
the Preview Window.
A description of each of the available features in the Subtract Background
dialog is given below.
Auto-scale
Clicking on the Auto-scale button will automatically adjust your Dark
Contrast and Background settings to optimal levels. You can then “fine tune”
these settings using the other controls.
Dark Contrast Slider
Adjusting the Dark Contrast prior to background subtraction allows you to
make fine distinctions in the amount of background present in your image.
Faint artifacts that may not be obvious to the unaided eye are revealed by
lowering the Dark Contrast level to close to the subtraction levels.
The Dark Contrast slider is similar to the High slider in the Transform dialog
box. Dragging the slider handle to the left will make faint signals appear
stronger. You can also move the slider incrementally by clicking on the slider
bar, or you can type a value into the field next to the slider.
Note: Adjusting the Dark Contrast slider by itself does not eliminate
background intensity; therefore, the OK button will not activate if you
only adjust this slider. If you only want to adjust the display contrast, and
not subtract background, use the Image > Transform function.