User guide

Personal Molecular Imager FX
10-5
You can also select the scanning area by entering coordinates in the
appropriate fields (Top, Bottom, Left, Right). After you enter a coordinate, the
position of the scanning area box will change accordingly.
When selecting, be sure to include the entire area of interest, and be generous
with borders. You can always crop the image later.
10.3 Step II. Select Resolution
The Personal FX acquisition window allows you to scan at 50, 100, 200, or 800
micrometers. These resolutions are listed as option buttons in the control
panel.
Fig. 10-4. Resolution option buttons.
The resolution you select should be based on the size of the objects (e.g.,
bands, spots) you are interested in. For example:
• 50 micrometer resolution should be reserved for images requiring the
highest level of detail, e.g., high density in situ samples, 1,536-well micro-
plates, high density arrays, samples with very closely spaced bands. Files
scanned at 50 micrometers can be very large.
• 100 micrometer resolution should be used for typical gels and arrays.
• 200 micrometer resolution is useful for gels with large bands and dot
blots.
• 800 micrometer resolution should be reserved for very large objects, such
as CAT assays.