User guide

Fluor-S MAX MultiImager
9-13
The progress of the dark exposure will be displayed in the Exposure Status
bar following the regular image exposure.
Note: In Normal mode, a dark image is only acquired the first time you perform
a scan with particular application and exposure settings. If you perform
subsequent scans with the same settings, no dark exposure will be taken.
Referenced
If you do not want to perform a dark exposure with each acquisition, you can
take a “reference” dark exposure that will be saved and subtracted from all
subsequent acquisitions. Click on the Referenced button to activate this
feature.
The first time you acquire an image after selecting this option, the Fluor-S
MAX will take a 300-second dark exposure that will be saved and used to
subtract the dark count from all subsequent acquisitions.
For image exposures of greater or less than 300 seconds, the reference dark
will be scaled accordingly and then subtracted. You can change the default
reference dark exposure time using the Reset Reference button (see below).
If you deselect the Referenced button and then reselect it, the old reference
dark exposure will still be available.
Note: Separate reference dark exposures will be taken for High Sensitivity
mode and Ultra Sensitivity mode. Once you have created a reference dark
in each of these modes, each reference dark will be used according to the
mode you are in.
Reset Reference Button
If you would like a reference dark with an exposure time that more closely
matches that of your typical scans, click on the Reset Reference button.