User guide

Fluor-S MAX MultiImager
9-9
The following table provides recommended exposure times for various
applications.
For most applications, you can select an exposure time, capture an image,
study it, then adjust the exposure time accordingly. Repeat this procedure as
many times as necessary to obtain a good image.
For chemiluminescent samples, which degrade over time and emit low levels
of light, you can select a high exposure time initially and use Live Acquire
mode to save intermediate exposures (see following section).
Recommended Exposure Times and Lenses
Sample
Recommended
Exposure
Lens & Filter
1
Accessories Used
Fluorescent Stain Gel 1–20 sec. Zoom/IR None
Fluorescence End-Label Gel 10 sec.–2 min. Zoom/IR None
Fluorescent Blot 0.1–3 sec. Zoom/IR None
Chemifluorescent Blot 0.1–3 sec. Zoom/IR None
Colorimetric Gel 1–5 sec. Zoom/IR White Diffusion Plate
Colorimetric Blot 0.2–10 sec. Zoom/IR None
X-ray film 0.1–5 sec. Zoom/IR White Diffusion Plate
Weak Chemiluminescence
2
2–10 min. 50 mm Chemi Tray
(if sample is small)
Strong Chemiluminescence
2
5 sec.–1 min. 50 mm Chemi Tray
(if sample is small)
1
For sharper focusing, close the f-stop down 1–2 stops from full open while focusing.
2
For chemi applications, the 50mm lens is recommended. Always remove the 660 filter.