User guide

Fluor-S MultiImager
8-7
Position
After you have selected your application, you are ready to center your gel or
other object within the camera frame. To do so, click on the Position button.
The Fluor-S will begin capturing a “live” image and updating it every second.
With the Position button selected, look at the image in the acquisition
window while you position your object in the center of the platen. If you have
a zoom lens on the camera, you can adjust the magnification while you
position.
You can select the Show Alignment Grid checkbox to facilitate positioning.
Fig. 8-6. Fluor-S alignment grid.
When you are finished positioning, click on the Stop button.
Focus
Note: Before focusing, you should adjust the f-stop on the camera to the lowest
setting (i.e., the maximum aperture). This reduces the depth of field,
allowing you to more accurately focus the camera. Then, after focusing,
increase the f-stop to the desired setting. (See the following table on
recommended f-stops.)
After you have positioned your sample, click on the Focus button and look at
the image in the acquisition window while aligning the two focusing arrows
on the camera lens. While focusing, the camera will limit its focus to a small