Product Manual

Page 7 of 15

Sofia Strep A FIA

8. Express as much liquid as possible from the Swab by squeezing the sides of the Tube as

the Swab is withdrawn.

Discard the Swab in accordance with your biohazard waste disposal protocol.

9. Fill the provided Yellow 100 µL Fixed Volume Pipette with the sample:

To fill the Fixed Volume Pipette with the sample:

a) FIRMLY squeeze the top bulb.

b) Still squeezing, place the Pipette tip into the sample.

c) With the Pipette tip still in the sample, release pressure on the bulb to ﬁll the Pipette.

10. Firmly squeeze the top bulb to empty the contents of the Fixed Volume Pipette into the

Cassette sample well. Extra liquid in the overﬂow bulb is OK.

NOTE: The Fixed Volume Pipette is designed to collect and dispense the correct amount

of patient sample. Discard the Pipette in your biohazard waste.

11. Proceed to the next section, “Using Soﬁa,” to complete the test.

USING SOFIA

WALK AWAY/READ NOW Modes

Refer to the Sofia User Manual for operating instructions.

Sofia may be set to two different modes (WALK AWAY and READ NOW). The procedures for each mode are described

below.

WALK AWAY Mode

In WALK AWAY Mode, the user immediately inserts the Cassette into Sofia. The user then returns after 5 minutes to get the

test result. In this mode, Sofia will automatically time the test development before scanning and displaying the test result.

READ NOW Mode

Allow the test to develop for the full 5 minutes BEFORE placing it into Sofia.

The user must first place the Cassette onto the counter or bench top for 5 minutes (outside of Sofia) and manually time

this development step. Then, the user inserts the Cassette into Sofia. In READ NOW Mode, Sofia will scan and display

the test result in less than 1 minute. Note: Results will remain stable for an additional 10 minutes after the recommended

development time of 5 minutes.

Pipette

Overflow

Sample

Squeeze

here

STREP A

Sample Well

Squeeze

here