Product Manual

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SofiaInfluenzaA+BFIA
  Page9of25
NOTE:Toobtainaccurateresults,avoidmucoidsubstanceswhen
fillingtheLarge,PinkFixedVolumePipettewithpatientsample
fromthecollectioncup.
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4. FirmlysqueezethetopbulbtoemptythecontentsoftheLarge,PinkFixed
VolumePipetteintotheReagentTube.Extraliquidleftoverintheoverflow
bulbshouldbeleftbehind.
NOTE:OncethesampleisaddedtotheReagentTube,vigorouslymixpriorto
addingthesampletothetestCassette.
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NOTE:TheFixedVolumePipettesaredesignedtocollectanddispensethe
correctamountofliquidsample.DiscardthePipetteinyourbiohazardwaste.
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5. FilltheprovidedSmall,Clear120µLFixedVolumePipettewithpatientsample
fromtheReagentTube,byslowlyreleasingpressureonthebulb.
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6. Firmlysqueezeth
etopbulbtoemptythecontentsoftheSm all,ClearFixed
VolumePipetteintotheCassettesamplewell.Extraliquidleftoverintheoverflow
bulbshouldbeleftbehind.DiscardthePipetteinyourbiohazardwaste.
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7. Promptlypro
ceedtothenextsection,“UsingSofia,”tocomp letethetest.
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USINGSOFIA
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Toobtainaccurateresults,SofiaandthetestCassetteshouldnotbeleftstandinginsidealaminarflowhood
orinaheavilyventilatedareaduringthe15‐minutedevel opmentstep.Theaccentuatedairflowcandrythe
sample and retard the flow of the sample through the test strip which can cause inaccurate or in
valid
results.
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Ifinfectionbyanovelvirusissuspectedorifyourinstitution’spolicyrequiresperformingrapidinfluenzatests
insidealaminarflowhood,afteraddingthesampletothetestCassette,coverthetestCassetteorinsertit
backintothefoilpouchuntilitisreadytoinsertin
toSofia.
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