Product Manual
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SofiaInfluenzaA+BFIA
Page8of25
4. RolltheswabheadagainsttheinsideoftheReagentTubeasyouremoveit.
Disposeoftheusedswabinyourbiohazardwaste.
5. FilltheprovidedSmall,Clear120µLFixedVolumePipettewiththepatientsample
fromtheReagentTube.
TofilltheFixedVolumePi
pettewiththepatientsample:
a. FIRMLYsqueezethetopbulb.
b. Stillsqueezing,placethePipettetipintothepatientsample.
c. WiththePipettetipstillinthepatientsample,slowlyreleasepress ure
onbulbtofillthePipette.
6. Firmlysqueezethetopbulbtoemptythecon
tentsoftheSmall,Clear120µL
FixedVolumePipetteintotheCassettesamplewell.Extraliquid leftoverinthe
overflowbulbshouldbeleftbehind.
NOTE:TheFixedVolumePipettesaredesignedtocollectanddispensethecorrect
amountofliquidsample.Discardthepipetteinyourbiohazardwaste.
7. Promptlypro
ceedtothenextsection,“UsingSofia,”tocomp letethetest.
NasopharyngealAspirate/WashorSamplesinViralTransportMediaTestProcedure
1. VerifythatSofiaissettothedesiredMode:WALKAWAYorREADNOW.
Seethe“UsingSofia”sectionformoreinformation.Alsoensurethatthe
liquidsampleisatroomtemperaturebeforeproceeding.
2. DispensealloftheReagentSolutionintotheReagentTube.SwirltheReagent
Tubetodiss
olveitscontents.
3. FilltheprovidedLarge,Pink250µLFixedVolumePipettewithpatientsample
fromthecollectioncuportesttube.
TofilltheFixedVolumePipettewiththesam ple:
a)FIRMLYsqueezethetopbulb.
b)Stillsqueezing,placethePipettetipintothepatientsample.
c)Withth
ePipettetipstillinthepatientsample,slowlyreleasepressure
onbulbtofillthePipette.