Product Manual

ManualsBrandsQuidel ManualsFlu/Influenza A+B TestSofia Influenza A+B FIA Test Kit

_________________________________________________________________________________

SofiaInfluenzaA+BFIA

  Page6of25

SAMPLECOLLECTIONANDHANDLING



SAMPLECOLLECTION

NasalSwabSample

Usethenasalswabsuppliedinthekit.

Tocollectanasalswabsample,carefullyinserttheswab(providedinthekit)intothenostrilthatpresentsthe

mostsecretionundervisualinspection.Usinggentlerotation,pushtheswabuntilresistanceismetatthelevel

oftheturbinates(lessthanonein

chintothenostril).Rotatetheswabseveraltimesagainstthenasalwallthen

removeitfromthenostril.



NasopharyngealSwabSample

Useanylonflockednasopharyngealswab,notsupplied.

To collect a nasopharyngeal swab sample, carefully  insert the swab into the nostril that presents the most

secretionundervisualinspection.Keeptheswabneartheseptumfloorofthenosewhilegentlypushingthe

swabintotheposteriornasopharynx.Rotatetheswabseveralti

mesthenremoveitfromthenasopharynx.



NasopharyngealAspirate/WashSampl e

Follow your institution’s protocol for obtaining nasopharyngeal aspirate/wash samples. Use the minimal

amount of saline that your procedure allows. Alternatively, if your institution does not provide a protocol,

thenconsiderthefollowingproceduresthatareusedbyclinicians:



To collect a nasopharyngeal aspirate sample: instill a few drops of sterile saline in

to the nostril to be

suctioned. Insert the flexible plasti c tubing along the nostril floor, parallel to the palate. After entering the

nasopharynx, aspirate the secretions while removing the tubing. The procedure should be repeated for the

othernostrilifinadequatesecretionswereobtainedfromthefirstnostril.



Tocollectanas

opharyngealwashsample:achildcouldsitintheparent’slapfacingforward,withthechild’s

headagainsttheparent’schest.Fillthesyringeoraspirationbulbwiththeminimalvolumeofsalinerequired

perthesubject’ssizeandage.Instillthesalineintoonenostrilwhiletheheadistiltedba

ck.Aspiratethewash

samplebackintothesyringeorbulb.Theaspiratedwashsamplewilllikelybeapproximately1ccinvolume.



Alternatively,followinginstillationofthesaline,tilt the head forward andletthe saline drain out

intoacleancollectioncup.

SAMPLETRANSPORTANDSTORAGE

Samplesshouldbetestedassoonaspossibleaftercollection.However,iftransportofsamplesisrequired,

minimaldilution,withviraltransportmedium(VTM),ofthesampleisrecommended,asdilutionmayresultin

decreasedtestsensitivity.Wheneverpossible,1milliliterorlessisbesttoavoidexcessivedilutionofthe

patien

tsample.

