Product Manual

Page 3 of 66QuickVue+ Strep A test
FOR INFORMATIONAL USE ONLY

FOR INFORMATIONAL USE ONLY
Not to be used for performing assay. Refer to most current package insert accompanying your test kit.
 Reagent B contains an acidic solution. If the solution contacts the skin or eye, flush
with large volumes of water.
 Do not use Reagent B if solution is green prior to mixing with Reagent A in the Tube.
If this occurs, contact Quidel Technical Support.
 To obtain accurate results, you must follow the Package Insert instructions.
SPECIMEN COLLECTION AND STORAGE
Collect throat swab specimens by standard clinical methods. When swabbing the throat,
be careful not to touch the tongue, sides or top of the mouth with the swab. Rub the
swab on the back of the throat, on the tonsils and in any other area where there is
redness, inflammation or pus. Bloody specimens can create an interfering background
and can cause an invalid result. Consult reference procedures such as the collection
method described by Facklam.
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Use rayon-tip or dacron-tip swabs with plastic shafts; individually packaged sterile
rayon-tipped swabs are provided in the kit. Do not use calcium alginate, cotton-tip or
wooden shafted swabs. Swab specimens should be processed as soon as possible after
collection. However, swabs can be stored in a clean, dry, sealable plastic tube or in 1 mL
or less liquid media, such as modified Stuart’s Transport Media, for up to eight hours at
room temperature (15–30°C) or 72 hours refrigerated (2–8°C). Do not use charcoal agar
or semi-solid transport media.
If culture results are also desired, lightly streak the swab on a 5% sheep blood agar
plate before using the QuickVue+ Strep A test. The culture plate must be streaked
prior to running the QuickVue+ test, as the reagents will kill the bacteria on the swab.
Throat swab specimens can also be obtained by dual swabs or by two swabs taken in
sequence for the culture procedure.
CULTURE CONFIRMATION
The QuickVue+ Strep A test can be used to confirm the identification of Group A
Streptococcus on SBA culture plates. Lightly touch a colony using a sterile swab.
Do not sweep the plate. Follow the instructions in the TEST PROCEDURE section
to test the sample.