Instruction Manual
Table Of Contents
- Intended Use
- Summary and Explanation
- Principles of the Procedure
- Materials Required (Provided)
- DTT solution 500mM
- SARS-CoV-2 PCR Mix
- Equipment and Supplies
- Warnings and Precautions
- Specimen Collection, Handling, and Storage
- Procedure
- For viscous samples (sputum and bronchial wash): Performed inside BSC 2
- NOTE: In the event that the test system becomes inoperable, notify supervision or designee for further direction. Patient specimens must be stored in a manner that maintains the integrity of the specimen.
- Quality Control
- Limitations
- Conditions of Authorization for the Laboratory
- Performance Characteristics
- References
8
MagNA Pure 96 Nucleic Acid Extraction
1.
Refer to PROC.129.01298, Nucleic Acid Isolation on the MagNA Pure 96 Instrument for general instructions on
using the MagNA Pure 96 Instrument
2.
All of the following steps are performed in the Specimen Preparation Area.
3.
Perform beginning of run maintenance on the MagNA Pure 96 instrument (as described in PROC.129.01298).
4.
In the Overview tab, select Enter Order, and select “External Lysis 450 µL ”.
5
The following parameters should be loaded:
MagNA Pure Kit Name: “DNA/Viral NA SV 2.0”
Protocol: “DNA BLOOD EXT LYS SV 3.1”
Sample volume: 450 µL
Elution volume: 50 µL
Internal Control should be “IPC”
6.
Click on the “…” button next to the IPC dropdown. Scan the barcode on the vial of internal control. Note the fill
volume (3.1mL) and number of samples in the batch. Set the expiration date.
7.
Set the total number of specimens and controls to match the current batch.
8.
Save the order to move to next screen.
9.
Refer to the software for the correct volumes and placement of the liquid reagents and disposable plastic
supplies. Label the sample elution cartridge with the batch ID.
10.
Carefully place the loaded sample cartridge on the MagNA Pure 96 instrument.
11.
Confirm proper placement on the screen.
12.
Start the run by clicking Start.
13.
At the completion of the run cover the sample elution cartridge with an adhesive plate sealer and transfer the
cartridge to the PCR set up area if processing immediately (within 30 minutes) or to frozen storage ( -70
o
C or
colder ) for up to one week.
14.
Tips and unused reagents may stay on the system for the next run. Cover reagents with a foil sealing cover if not
using immediately.
15.
Perform end of run maintenance on MagNAPure 96 instrument as in PROC.129.01298.