Instruction Manual

4
DTT solution 500mM
Add 100 µL of nuclease free water to one microtube containing DTT and mix with pipette tip. Add the entire 100µL DTT
solution into 5mL of cold sterile 0.01 M PBS (pH 7.2) and mix briefly. Discard any unused reconstituted DTT
SARS-CoV-2 PCR Mix
Combine the ingredients in the amounts listed below.
Dispense in 455 µL aliquots label as: NCOV PCR Mix
Lot#/Prep: (preparation date. initials)
Exp. date: (1 year from preparation date)
Store at -60 to -90°C
Each aliquot is sufficient for up to 48 reactions.
Storage Store at -60 to -90°C.
Stability Expires 1 year after preparation.
See Formulation Sheet EFORM.129.001481.
Item
µL per
reaction
Unit of Measure
for 1,000 reactions
Final Concentration
per 25 µL reaction
Sterile Nuclease Free Water
3.75
3.75 mL
---
2019-nCoV_N1 Forward Primer (10 µM in TE, pH 8.0)
1.00
1.00 mL
0.4 µM
2019-nCoV_N1 Reverse Primer (10 µM in TE, pH 8.0)
1.00
1.00 mL
0.4 µM
2019-nCoV_N1 Probe (10 µM in TE, pH 8.0)
0.25
0.25 mL
0.1 µM
2019-nCoV_N3 Forward Primer (10 µM in TE, pH 8.0)
1.00
1.0 mL
0.4 µM
2019-nCoV_N3 Reverse Primer (10 µM in TE, pH 8.0)
1.00
1.0 mL
0.4 µM
2019-nCoV_N3 Probe (10 µM in TE, pH 8.0)
0.25
0.25 mL
0.1 µM
50X Exogenous NA Primer/Probe Mix
0.50
0.50 mL
1X
Total
8.750 µL
8.750 mL
Equipment and Supplies
• Applied Biosystems 7500 Real Time PCR System (or ABI 7500 fast system run as a standard ABI 7500)
• Roche MagNA Pure 96 System (Magna Pure extraction)
• Hamilton MagEx STAR (Omega extraction)
• Bench-top centrifuge
• Serological Pipet (Pipette Aid)
• Sterile screw cap 15 mL conical tubes
• Sterile screw cap 50 mL conical tubes
• P10, P20, P200, P1000 pipettes
• P-10, P-20, P-200, P-1000 ART Plugged Tips
• 1.5 mL or 2 mL microcentrifuge tubes
• Metal tubes
• Standard absorbent wipes
• Latex gloves and other protective equipment (see Procedure)
• Biohazard Absorbent Wipes
• 96-Well Optical Reaction Plate
• Optical Adhesive Cover
• Vortexer
• Microcentrifuge
Self-collection Kits
Quest Diagnostics Self-collection kit for COVID-19
Warnings and Precautions
1. For in vitro diagnostic use (IVD).
2. This test has not been FDA cleared or approved; the test has been authorized by FDA under an Emergency Use