Instruction Manual
Table Of Contents
- Intended Use
- Summary and Explanation
- Principles of the Procedure
- Materials Required (Provided)
- DTT solution 500mM
- SARS-CoV-2 PCR Mix
- Equipment and Supplies
- Warnings and Precautions
- Specimen Collection, Handling, and Storage
- Procedure
- For viscous samples (sputum and bronchial wash): Performed inside BSC 2
- NOTE: In the event that the test system becomes inoperable, notify supervision or designee for further direction. Patient specimens must be stored in a manner that maintains the integrity of the specimen.
- Quality Control
- Limitations
- Conditions of Authorization for the Laboratory
- Performance Characteristics
- References
10
Omega Method using the Hamilton STAR Nucleic Acid Isolation
1. Refer to PROC.129.01744, Hamilton MagEx STAR Autoload System Use and Maintenance for general
instructions on using the Hamilton STAR.
2.
All of the following steps are performed in the Specimen Preparation Area.
3.
Perform daily maintenance on the Hamilton instrument.
4. In the Method Launcher desktop application located on the desktop, click on the “Omega extraction”
tab, and select the number of plates to be run (up to 4 plates). If a partial plate is run, the instrument will
process as full.
5.
Refer to the software for the correct volumes and placement of the liquid reagents and disposable
plastic supplies. Label the sample elution cartridge with the batch ID.
6.
Confirm proper placement on the screen.
7.
Start the run by clicking the green arrow located in the upper left-hand corner of the application .
8
Double check the accuracy of the matching load number across the extraction and output plates.
9.
At the completion of the run cover the sample elution plate with an adhesive plate sealer and store at 2-
8°C until needed for PCR set-up.
10.
Tips and unused reagents may stay on the system for the next run. Cover reagents with a foil sealing
cover if not using immediately.
11.
Perform end of run maintenance on the Hamilton STAR.
Start the UV Decontamination by clicking on the “StarUVLight Software” prompt located on the desktop.
Set time for 30 minutes between each run.
The UV Decontamination must be performed once each 8-hour shift for a full 60 minutes on instruments
used for the SARS-CoV-2 assay.