User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover

Prerequisites
This guide also assumes that you have:
• Knowledge of techniques for handling and preparing samples for PCR.
• A general understanding of data storage, file transfers, and copying and pasting.
Instrument hardware
The QuantStudio
®
3D Digital PCR Instrument consists of the components shown in
the following figure.
1
2
3
4
5
6
7
8
1
Touchscreen – Provides access to the
instrument functions, such as data
transfer and instrument operation.
2
Chip tray – Conveys the QuantStudio
®
3D
Digital PCR 20K Chip to and from the
imaging stage in the interior of the
instrument.
3
USB port – Provides USB communication
with the QuantStudio
®
3D Instrument. Can
be used to transfer data to and from the
instrument.
4
Fuse cover – Dual 1.6A, Time Lag T, 250
VAC, 5 × 20-mm electrical fuses that
protect the instrument from excessive
electrical current.
5
Power switch – Power switch for the
instrument, where the states are on ( | )
or off ( O ).
6
Power port – A 100–240 VAC port that
provides power to the QuantStudio
®
3D
Instrument.
7
Ethernet port – An RJ45 port that provides
Ethernet (gigabit) communication with the
QuantStudio
®
3D Instrument.
Instrument software
The QuantStudio
®
3D Digital PCR Instrument includes a software suite used to
analyze and manage digital PCR data generated by imaging
QuantStudio
®
3D Digital
PCR 20K Chips. The QuantStudio
®
3D AnalysisSuite
™
Software features a server, and
cloud deployment that can satisfy a broad range of laboratory requirements.
Note: For more information on the AnalysisSuite
™
Software, refer to the
AnalysisSuite
™
Software Help which is available from any point in the software by
clicking the ? button.
About this guide
Prerequisites
QuantStudio
®
3D Digital PCR System User Guide
9