User guide

Observation Possible Cause Action
Condensation may have been
present on the Digital PCR 20K
Chip during imaging.
IMPORTANT! Condensation
may not be visible on the Digital
PCR 20K Chip.
• If condensation is
not visible
– If possible, read the Digital
PCR 20K Chip again after it
has warmed to room
temperature. Once
equilibrated, the data quality,
flag, and separation of
amplified versus non-
amplified peaks should
improve noticeably.
• If condensation is visible –
Using a low-lint wipe,
remove any condensation or
Immersion Fluid from the
surface of the chip by wiping
in one direction. If
necessary, use a low-lint
wipe sprayed with
isopropanol to remove any
dried residue.
The Sample Loading Blade was
not seated correctly within the
loader arm.
Before pressing the loading
button, confirm that the Sample
Loading Blade is firmly seated
and flush against the loader arm.
Chapter 5 Troubleshooting
Troubleshooting chip images using the Chip View
5
QuantStudio
®
3D Digital PCR System User Guide 83