User guide

Observation Possible Cause Action
Chip was not completely covered
with Immersion Fluid
immediately after loading (while
still on the chip loader or heated
block)
Note: (QuantStudio
®
3D
Instrument firmware version 1.1
or less) Yellow around the edges
is a strong indication of
evaporation.
Add Immersion Fluid as soon as
the loading blade is off of the
chip.
Ensure that entire chip is covered
with Immersion Fluid, even the
edges.
Note: When adding Immersion
Fluid, the fluid can overflow into
the chip base, but do not allow it
to spread onto the sides of the
chip lid.
Immersion Fluid was not applied
to the chip immediately after
loading (evaporation of the PCR
reaction).
Debris or other contaminants are
present on the Chip Sealant
during imaging.
No action required.
The AnalysisSuite
™
Software can
compensate for small quantities
of debris or other contaminants
on the Digital PCR 20K Chip.
Chapter 5 Troubleshooting
Troubleshooting chip images using the Chip View
5
82 QuantStudio
®
3D Digital PCR System User Guide