User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover

Observation Possible Cause Action
A bubble was present in the
Sample Loading Blade when it
was used to apply the PCR
reaction to the Digital PCR 20K
Chip.
If possible, use the
AnalysisSuite
™
Software to filter
the low quality data points, or
discard the chip and run the
sample again.
When loading the Sample
Loading Blades:
• If you are using a manual
pipette, pipette to the first
stop.
• Decrease your pipetting
speed.
Chapter 5 Troubleshooting
Troubleshooting chip images using the Chip View
5
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QuantStudio
®
3D Digital PCR System User Guide