User guide

Table Of Contents

About this guide

CAUTION! ABBREVIATED SAFETY ALERTS. Hazard symbols and hazard

types specified in procedures may be abbreviated in this document. For the

complete safety information, see the “Safety” appendix in this document.

IMPORTANT! Before using this product, read and understand the information in the

“Safety” appendix in this document.

Revision history

Revision

Date Description

01 03, 2013 Initial version

02 06, 2013 Updated general chip preparation and instrument networking.

A.0 12, 2013 Updated the manual chip preparation and added procedures for

chip preparation using the QuantStudio

3D Digital PCR Chip

Loader and wireless network installation.

B.0 04, 2014 Added support for the ProFlex

™

2x Flat PCR System and revised

the Digital PCR 20K Chip loading instructions.

C.0 02, 2015 Updated for firmware and software revisions, including changes to

instrument networking, thermal cycling, chip analysis,

maintenance, software access, and computer requirements.

Updated to latest corporate boilerplate. Updated general sample

and chip preparation, system installation, troubleshooting, and

parts and materials.

Purpose

This user guide provides step-by-step instructions for preparing and loading samples

onto QuantStudio

3D Digital PCR 20K Chips, thermal cycling the prepared

consumables using a

ProFlex

™

2x Flat PCR System (or Dual Flat Block GeneAmp

PCR System 9700), and analyzing the consumables using the QuantStudio

3D Digital

PCR Instrument.

WARNING! The protection provided by the equipment may be impaired if the

instrument is operated outside the environment and use specifications, the user

provides inadequate maintenance, or the equipment is used in a manner not

specified by the manufacturer (Thermo Fisher Scientific).

QuantStudio

3D Digital PCR System User Guide