User guide

Troubleshooting
Troubleshooting chip images using the Chip View
The following table summarizes some of the more common problems that can affect
imaging of Digital PCR 20K Chips. In the examples shown below, the conditions
triggered a data flag and were diagnosed using the Chip View of the AnalysisSuite
™
Software.
Observation Possible Cause Action
• Excess PCR reaction was
present on the Digital PCR
20K Chip after loading it with
the Sample Loading Blade.
• (Manual load only) The
Sample Loading Blade was
drawn across the chip too
quickly or at an angle
shallower than 70-80°.
If possible, use the
AnalysisSuite
™
Software to filter
the low quality data points, or
discard the chip and run the
sample again.
To prevent leaving excess PCR
reaction on the Digital PCR 20K
Chips:
• Make sure to install theTilt
Base to the thermal cycler.
You must thermal cycle the
Digital PCR 20K Chips at an
11° angle.
• (Manual load only) Confirm
that the heated block used to
load Digital PCR 20K Chips is
set to 40±1°C.
• (Manual load only) Make
sure to wait 20 seconds after
loading a Digital PCR 20K
Chip before pre-wetting it
with Immersion Fluid.
• (Manual load only) Make
sure to draw the Sample
Loading Blade across the
chip slowly (>10 seconds)
and at a 70-80° angle.
5
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®
3D Digital PCR System User Guide 79