User guide

The QuantStudio
®
3D Digital PCR Instrument displays a summary of the imaging
data after each successful run. For each QuantStudio
®
3D Digital PCR 20K Chip
imaged, the instrument displays the following information:
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7
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2
1
Chip ID – Displays the ID number of the
Digital PCR 20K Chip. If the instrument
cannot read the chip ID, the touchscreen
displays the date and time of the run.
2
Date/time stamp – Displays the date and
time of the run in <
yymmdd >_< hhmmss >
format.
3
FAM/VIC copy number estimate – Displays
the estimated concentration in copies per
µL calculated from the fluorescence
signal associated with the amplification of
the nucleic acid sequence targeted by the
FAM
™
and/or VIC
®
dye-labeled probes.
Beside the copy number estimate, the
QuantStudio
®
3D Instrument displays a
flag icon, color-coded to indicate the
status of the preliminary analysis of the
image data for the associated probe.
4
Network status icon – Represents the
status of the QuantStudio
®
3D Instrument
network connection:
• – Wired (Ethernet) connection
selected; connected to network.
• – Wired (Ethernet) connection
selected; no connection available.
• – Wireless connection selected;
connected to network.
• – Wireless connection selected; no
connection available.
5
Data destination icon – Represents the
selected data destination:
• – Cloud destination selected.
• – USB drive destination selected.
• – Network share destination
selected.
6
File name – Name of the experiment
(.eds) file created for the imaged Digital
PCR 20K Chip.
Note: After the analysis, the instrument
copies the file to the selected destination
and stores the file locally.
7
Scroll buttons – Allow you to display the
results of previously imaged Digital PCR
20K Chips when running them in a series.
About the results
screen
Chapter 4 Analyze the Prepared Chips
Analyzing the data
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74 QuantStudio
®
3D Digital PCR System User Guide