User guide

5.
After reviewing the results of the run, open the chip tray and remove the chip (or
remove the USB drive, if present) to close the results screen.
If the analysis of the imaging data produced a (red) for either probe (FAM
™
or
VIC
®
dye), visually inspect the chip for problems and read the chip again.
Note: If the run fails because the instrument is unable to read the Digital PCR
20K Chip, load the chip again to repeat the reading. If the run fails repeatedly,
discard the chip and prepare a replacement.
After a Digital PCR 20K Chip has been read:
• You can read the imaged Digital PCR 20K Chip again for up to 1 hour after
thermal cycling.
• If you read multiple Digital PCR 20K Chips in rapid succession, touch the scroll
buttons ( / ) to review the results of the previously imaged chips.
•
The QuantStudio
®
3D Instrument retains a copy of the analyzed data for the
imaged Digital PCR 20K Chip that you can access from the Run History screen.
Note:
The results will remain in the QuantStudio
®
3D Instrument cache for
approximately 600 readings. After 600 chips, the instrument removes the oldest
exported data file in the cache to store each new reading.
IMPORTANT!
Confirm that the QuantStudio
®
3D Instrument is unloaded before you power off and
move the instrument. If the instrument is moved while loaded, the
Digital PCR 20K
Chip inside may become dislodged from the chip tray and obstruct the chip tray
mechanism.
Analyzing the data
After the QuantStudio
®
3D Digital PCR Instrument images a Digital PCR 20K Chip
,
the instrument performs an initial evaluation of the raw data to determine the
viability of the collected data. During the analysis, the instrument identifies the wells
within the captured images and performs a quality assessment of the composite
results to determine whether the instrument has collected usable data. If the imaging
data pass the initial quality assessment, the QuantStudio
®
3D Instrument calculates
the estimated concentration of the nucleic acid sequence targeted by the FAM
™
and
VIC
®
dye-labeled probes. The resulting data are reported in copies per μL along with
the results of the quality assessment displayed using colored flags.
About the analysis
Chapter 4 Analyze the Prepared Chips
Analyzing the data
4
QuantStudio
®
3D Digital PCR System User Guide 73