User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover

Analyze the chips
The following materials are required to image loaded Digital PCR 20K Chips on the
QuantStudio
®
3D Digital PCR System.
• Prepared Digital PCR 20K Chip (from the previous section)
• Isopropanol
• Gloves, powder-free, nitrile
• Cleanroom-grade, low-lint polyester wipes
For optimal efficiency when running large numbers (>6) of Digital PCR 20K Chips,
follow the guidelines below:
• Run the Digital PCR 20K Chips in alphanumeric order (according to chip ID) to
help avoid mistakes when entering sample information.
• If you are running multiple chips, you can run the next chip in the series when
the touchscreen displays the Analyzing Chip screen .
To prepare the instrument for imaging data, edit data destination and file name
settings before loading a chip into the instrument.
1.
Set the destination for the imaging data:
a.
From the Main Menu of the QuantStudio
®
3D Instrument, touch , then
touch Data Destinations.
b.
In the Data Destinations screen, touch the desired destination for the
imaging data.
You can configure the QuantStudio
®
3D Instrument to upload the imaging
data to a USB drive, a network file server, or the cloud data storage service.
Materials
required
Guidelines for
high-throughput
analysis
Prepare the
instrument for
imaging data
Chapter 4 Analyze the Prepared Chips
Analyze the chips
4
QuantStudio
®
3D Digital PCR System User Guide
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