User guide

Table Of Contents

Analyze the Prepared Chips

■

Using the QuantStudio

3D Digital PCR Instrument ...................... 66

■

Analyze the chips .................................................... 69

■

Analyzing the data ................................................... 73

■

Using the QuantStudio

3D AnalysisSuite

™

Software .................... 77

Using the QuantStudio

3D Digital PCR Instrument

The QuantStudio

3D Digital PCR Instrument performs the image capture and

subsequent preliminary analysis of QuantStudio

3D Digital PCR 20K Chips

following thermal cycling. The QuantStudio

3D Instrument processes Digital PCR

20K Chips individually, where each read lasts approximately one minute. After

reading a chip, the instrument performs an initial evaluation of the imaging data

collected during the run and displays the results on the instrument touchscreen.

QuantStudio

3D System data flow

For each imaged Digital PCR 20K Chip, the QuantStudio

3D Instrument generates

and saves a single experiment (.eds) file that contains the processed imaging data and

the results from the preliminary analysis on the instrument. Depending on the chosen

data destination, the instrument transfers the saved imaging data directly to the Life

Technologies

™

cloud for immediate access by the AnalysisSuite

™

Software, or to a

network file server or local USB drive for later manual import.

QuantStudio™ 3D

AnalysisSuite™ Software

About imaging and

primary analysis

QuantStudio

3D Digital PCR System User Guide