User guide

3.
Remove the Chip Adapters from the thermal cycler sample block and place them
on a clean, dry surface. Remove the Digital PCR 20K Chips from the Chip
Adapters and allow them to equilibrate to room temperature.
Note: To remove a chip from a Chip Adapter, push the Digital PCR 20K Chip
from beneath and grasp the edges of the Chip Case, then remove it.
IMPORTANT! Handle the Chip Cases gently, being careful to not apply pressure
to the Digital PCR 20K Chips within them.
4.
Visually inspect each Digital PCR 20K Chip for signs of leaks or potential
problems. Using a low-lint wipe, remove any condensation or
Immersion Fluid
from the surface of the chip by wiping in one direction. If necessary, use a low-
lint wipe sprayed with isopropanol to remove any dried residue.
IMPORTANT! Thoroughly clean each Chip Case before proceeding.
The Digital PCR 20K Chip is now ready to be imaged by the QuantStudio
®
3D
Instrument. Image the Digital PCR 20K Chip
s within 1 hour after removing them
from the thermal cycler.
IMPORTANT! Store the prepared Digital PCR 20K Chip
s in a clean, dry, dark location
until you are ready to image them. Prolonged exposure to light can diminish the
fluorescence of the photosensitive dyes in the chips.
Note: If a Digital PCR 20K Chip is sealed improperly, the chip may leak and
contaminate the sample block. If you suspect that a chip has leaked, remove the fluid
from the sample block using a low-lint wipe sprayed with isopropanol.
Chapter 3 Perform the PCR
Thermal cycle the Digital PCR 20K Chips
3
QuantStudio
®
3D Digital PCR System User Guide 65