User guide

Table Of Contents

Use the thermal cycler to select and start the pre-programmed run for the Digital

PCR 20K Chips. See the ProFlex

™

PCR System User Guide (Pub. no. MAN0007697)

or the GeneAmp

PCR System 9700 Base Module User Manual (Pub. no. 4303481) for

more information on running methods.

You can remove the Digital PCR 20K Chips from the block immediately after the

final extension step at 60°C is complete and the temperature of the block is

<25°C.

IMPORTANT! Read the Digital PCR 20K Chips within one hour after you

remove them from the sample block. After thermal cycling, the Digital PCR 20K

Chips can remain on the sample block for up to 24 hours at 10°C.

Note: If you programmed your thermal cycler to perform a 10°C hold following

thermal cycling, do the following to prevent condensation:

Confirm the final extension step at 60°C is complete, then stop the run:

ProFlex

™

System – Press Stop Run, then press OK.

GeneAmp

PCR System 9700 – Press STOP twice , then press Exit.

2. Allow the thermal cycler to sit for at least 10 minutes with the heated lid

closed.

3. Remove the chips.

Use a low-lint wipe sprayed with isopropanol to remove any remaining

condensation or immersion fluid from the lids before imaging them.

CAUTION! PHYSICAL INJURY HAZARD. During operation, the sample

block can be heated to 100°C. Before performing the following procedure, be

sure to wait until the sample block reaches room temperature.

Open the heated cover to expose the Digital PCR 20K Chip

Remove the QuantStudio

Thermal Pads from the sample block and set them on

a clean, dry surface.

IMPORTANT! Do not discard the

QuantStudio

Thermal Pads. They can be

reused multiple times.

Unload the

thermal cycler

Chapter 3 Perform the PCR

Thermal cycle the Digital PCR 20K Chips

QuantStudio

3D Digital PCR System User Guide