User guide

Table Of Contents

Load the QuantStudio

3D Digital PCR 20K Chips into the thermal cycler:

Place the sealed Digital PCR 20K Chips onto the sample block so that the fill

ports on the chips are positioned toward the front of the thermal cycler. The

fill port must be elevated during thermal cycling to prevent leaks from

improperly sealed chips.

IMPORTANT! If you are thermal cycling less than 24 Digital PCR 20K

Chips, load according to the following guidelines:

Load each Digital PCR 20K Chip so that the fill port is oriented toward

the front of the thermal cycler.

X0300036

Front of instrument

CAUTION HOT

24x

Load the right sample block first, placing at least 1 chip on the right

sample block.

Balance the load between the left and right blocks so that the pressure

applied by the heated cover and thermal pads is uniform across all of the

loaded chips.

Lay the QuantStudio

Thermal Pad

s over the Digital PCR 20K Chips.

IMPORTANT! Make certain that each QuantStudio

Thermal Pad is

centered on the sample block and that it completely covers the Digital PCR

20K Chips.

IMPORTANT! Do not allow the QuantStudio

Thermal Pads to become

dirty. Fluids, debris, and other contaminants that adhere to the pad can

transfer to the

Digital PCR 20K Chips during thermal cycling and fluoresce

during imaging.

CAUTION HOT

Close and engage the heated cover with the QuantStudio

3D Digital PCR

20K Chip

IMPORTANT! Verify the heater cover is completely engaged to ensure a

proper seal.

Chapter 3 Perform the PCR

Thermal cycle the Digital PCR 20K Chips

QuantStudio

3D Digital PCR System User Guide