User guide

To ensure the proper function of your loaded Digital PCR 20K Chips, handle the Chip
Cases according to the following guidelines:
•
Always wear powder-free gloves when handling Chip Cases.
IMPORTANT! Never handle the Chip Cases without gloves. Oils from your
hands can contaminate the Digital PCR 20K Chips and interfere with imaging.
• Handle the loaded Digital PCR 20K Chips by gently gripping the sides of each
Chip Case, being careful to not apply pressure to the Digital PCR 20K Chip
within it.
• Do not touch the surface of the QuantStudio
®
3D Digital PCR Chip Case Lid.
If you accidentally touch the surface of a Chip Case Lid, clean the Chip Case
using a laboratory wipe that has been thoroughly sprayed with isopropanol, then
dry it by wiping in one direction using a clean low-lint wipe.
Note: Debris or excess sample on the Chip Case Lid can interfere with thermal
uniformity during thermal cycling, or fluoresce during imaging.
• Thermal cycle loaded Digital PCR 20K Chips within 2 hours after loading them.
X0300036
Note: Install the Tilt Base to the thermal cycler for the following procedure.
CAUTION! PHYSICAL INJURY HAZARD. During operation, the sample
block can be heated to 100°C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
IMPORTANT! Thermal cycle prepared QuantStudio
®
3D Digital PCR 20K Chips
within 2 hours after loading them.
IMPORTANT! Thermal cycling of Digital PCR 20K Chips must be performed on a
ProFlex
™
2x Flat PCR System or a Dual Flat Block GeneAmp
®
PCR System 9700 as
described in this guide. No other thermal cyclers can be substituted at the time of the
release of this manual.
1.
Open the heated cover, then wipe the surface of both sample blocks using a low-
lint wipe to ensure that they are clean and dry.
2.
Confirm the Tilt Base
is installed to the thermal cycler and that Chip Adapters
are installed to both sample blocks.
IMPORTANT! You must install Chip Adapters to both sample blocks when
thermal cycling
Digital PCR 20K Chips, even if you are using only one block.
Guidelines for
handling Chip
Cases
Perform the PCR
Chapter 3 Perform the PCR
Thermal cycle the Digital PCR 20K Chips
3
62 QuantStudio
®
3D Digital PCR System User Guide