User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover
Table 5 GeneAmp
®
PCR System 9700 PCR Method
PCR Protocol
Run
Speed
Reaction
Volume
Stage 1 Stage 2 Stage 3
96.0°C 60.0°C 98.0°C 60.0°C 10.0°C
[1]
Standard 20 μL10:00 2:00 0:30 2:00 99:59
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
O
ptional step.
Thermal cycle the Digital PCR 20K Chips
The following materials are required to thermal cycle prepared QuantStudio
®
3D
Digital PCR 20K Chips for use with the QuantStudio
®
3D Digital PCR System.
• Prepared QuantStudio
®
3D Digital PCR 20K Chips (from the previous section)
• ProFlex
™
2x Flat PCR System (or Dual Flat Block GeneAmp
®
PCR System 9700)
• QuantStudio
®
3D Digital PCR Chip Adapters
• QuantStudio
®
3D Digital PCR Thermal Pads
• QuantStudio
®
3D Digital PCR Tilt Base
• Isopropanol
• Gloves, powder-free, nitrile
• Cleanroom-grade, low-lint polyester wipes
Materials
required
Chapter 3 Perform the PCR
Thermal cycle the Digital PCR 20K Chips
3
QuantStudio
®
3D Digital PCR System User Guide
61