User guide

• Confirm the thermal cycler is running the correct firmware version number to
thermal cycle Digital PCR 20K Chip
s:
– ProFlex
™
2x Flat PCR System – The version number displayed in the About
Instrument screen must be 1.1.4 or greater.
1.1.4 or greater
About Instrument
Wired IP Address
Wireless IP Address
Instrument Serial Number
Block Serial Number
Firmware Version
10.0.2.15
12345678
12345678
1.1.4
b1765c31bd08370ba632d35ea56c505b
UUID
Note: If the ProFlex
™
2x Flat PCR System version is less than 1.1.4, see the
ProFlex
™
PCR System User Guide (Pub. no. MAN0007697) for information on
upgrading the instrument firmware before thermal cycling
Digital PCR 20K
Chips.
– GeneAmp
®
PCR System 9700 – The version number displayed in the main
menu must be 3.12 or greater.
08:00 AM 4/25/13 25.0°C
GeneAmp® PCR System 9700
Name:tc001 User:<<pe>>
F1 F3 F4 F5F2
Run UserUtilEditCreate
Version: 3.12
3.12 or
gr
eater
Note: If the GeneAmp
®
PCR System 9700 version is less than 3.12, contact
technical support to upgrade the instrument firmware before thermal cycling
Digital PCR 20K Chip
s (see “Customer and technical support“ on page 121).
• Confirm that the thermal cycler is programmed with the method for thermal
cycling QuantStudio
®
3D Digital PCR 20K Chips. If missing, create the PCR
method before proceeding.
Note: See the ProFlex
™
PCR System User Guide (Pub. no. MAN0007697) or the
GeneAmp
®
PCR System 9700 Base Module User Manual (Pub. no. 4303481) for more
information on programming methods.
Table 4 ProFlex
™
2x Flat PCR System PCR Method
PCR Protocol
Cover
Temp.
Reaction
Volume
Stage 1 Stage 2 Stage 3
96.0°C 60.0°C 98.0°C 60.0°C 10.0°C
[1]
70.0°C 1 nL0:10:00 0:02:00 0:00:30 0:02:00 ∞
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
O
ptional step.
Chapter 3 Perform the PCR
Choosing a thermal cycler
3
60 QuantStudio
®
3D Digital PCR System User Guide