User guide

Table Of Contents

Before running each batch of Digital PCR 20K Chips, perform the following to ensure

efficient thermal cycling:

•

Confirm that the following parts are installed or available for use:

Confirm that clean QuantStudio

3D Digital PCR Thermal Pads are available

for use.

IMPORTANT! If a QuantStudio

Thermal Pad becomes dirty, clean the pad

using a low-lint wipe sprayed with isopropanol, then let it dry. Fluids,

debris, and other contaminants that adhere to the pad can transfer to the

Digital PCR 20K Chip

s during thermal cycling and fluoresce during

imaging.

IMPORTANT! Use only QuantStudio

Thermal Pads that are approved for

use with Digital PCR 20K Chip

s. Thermal compression pads from other PCR

applications may not apply uniform pressure or heat distribution to all chips

on the sample block.

Confirm that a QuantStudio

3D Tilt Base is installed beneath the thermal

cycler.

IMPORTANT! The Tilt Base inclines the sample block by 11°, the angle

required for the optimal performance of the Digital PCR 20K Chips.

11°

Open the heated cover and confirm that QuantStudio

3D Digital PCR Chip

Adapters are installed to both sample blocks.

Note: Each

Chip Adapter includes a set of four alignment pegs that fit into

the holes of a flat sample block. The Chip Adapter can fit onto either side of

the sample block (right or left).

CAUTION HOT

Prepare the

thermal cycler

Chapter 3 Perform the PCR

Choosing a thermal cycler

QuantStudio

3D Digital PCR System User Guide