User guide

10.
Visually inspect the sealed Digital PCR 20K Chip for potential problems:
• Leaks – Confirm that no Immersion Fluid is leaking from the fill port or
around the seal between the Chip Case and lid.
• Bubbles – Confirm that the Chip Case is free of excessive bubbles. One small
air bubble is acceptable.
• Correct lid orientation – Confirm that the Chip Case Lid and the Digital
PCR 20K Chip are correctly aligned.
If the loaded and sealed Digital PCR 20K Chip fails any of the criteria above,
discard it and prepare a duplicate chip. Once sealed, the Chip Case cannot be
opened and resealed.
IMPORTANT! Handle the Chip Cases gently, being careful to not apply pressure
to the Digital PCR 20K Chips within them.
Thermal cycle the prepared Digital PCR 20K Chips within 2 hours after loading them.
IMPORTANT! Store the prepared Digital PCR 20K Chips in a clean, dry, dark location
until you are ready to load them onto the thermal cycler.
Note: If desired, use a permanent pen or marker to label the back of the Digital PCR
20K Chip. Marking the back of the chips will not affect the imaging data.
Chapter 2 Prepare Samples and Load Reactions
Load chips manually
2
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3D Digital PCR System User Guide 57