User guide

Table Of Contents

Seal the Digital PCR 20K Chip:

Remove the red protective film from the QuantStudio

3D Digital PCR Chip

Case Lid, then carefully place it onto the prepared Digital PCR 20K Chip so

that the window in the lid aligns with the chip.

IMPORTANT! Before applying the Chip Case Lid, make certain that it is

correctly oriented to the Digital PCR 20K Chip assembly.

Pinch together all four sides and corners of the

Chip Case for at least

10 seconds to seal it. While pressing, apply some lateral pressure to ensure a

tight seal.

Note: You can remove the Digital PCR 20K Chip from the loading surface

when you apply pressure to seal it.

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>10 sec.

Fill the Chip Case with Immersion Fluid:

Hold the Chip Case by its edges and at a 45° angle so that air can escape

from the loading port as you fill it, then carefully dispense Immersion Fluid

into the port until the

Chip Case contains an air bubble no larger than the fill

port (<2-3 mm in diameter).

After filling the Immersion Fluid, rotate it slightly to reveal any air bubbles

that might be hidden behind the serial number label. If bubbles are present,

add additional Immersion Fluid to remove them.

IMPORTANT! Expel the Immersion Fluid slowly. If injected too quickly, the

fluid can flush the reactions suspended within the chip.

Note: Minimize air bubble creation when you dispense the fluid; however,

one small air bubble (<2-3 mm in diameter) is acceptable.

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Using a low-lint wipe, remove any excess

Immersion Fluid from the Chip

Case to ensure optimal imaging on the QuantStudio

3D Instrument.

Chapter 2 Prepare Samples and Load Reactions

Load chips manually

QuantStudio

3D Digital PCR System User Guide