User guide

Table Of Contents

While holding the Sample Loading Blade at a 70-80° angle relative to the

heated block so that the port faces up, place the edge of the blade at the end

of the Digital PCR 20K Chip. Adjust the angle of the blade until you visually

confirm that it is wetting the chip. Then, in one smooth motion, slowly drag

the blade across the chip while applying a slight downward pressure to

dispense the reaction.

IMPORTANT! Move the Sample Loading Blade slowly. The time required

to move the blade across the chip should be approximately 10-12 seconds.

IMPORTANT! Before you draw the blade across the chip, visually confirm

that the

Sample Loading Blade is wetting the chip. If not, adjust the angle

slightly.

40±1°C

70-80°

10 sec.

Confirm that the Sample Loading Blade contains no residual fluid,

indicating that the reaction has been completely loaded onto the chip.

IMPORTANT! Do not apply the Sample Loading Blade

to the Digital PCR

20K Chip more than once.

Allow the Digital PCR 20K Chip to sit on the heated block for 20 seconds

while the PCR reaction stabilizes within the chip.

Add several drops of

Immersion Fluid directly to the chip so that the fluid

covers the entire surface.

IMPORTANT! Do not allow the syringe tip to contact the

Digital PCR 20K

Chip.

Correct

Fluid on

edges

Incorrect

Insufficient

fluid

Fluid over

chip

Note: A small amount of fluid running off the surface of the chip is

acceptable.

Chapter 2 Prepare Samples and Load Reactions

Load chips manually

QuantStudio

3D Digital PCR System User Guide