User guide

Table Of Contents

Remove the following consumables from their packaging and place them on a

clean, dry, lint-free surface:

• QuantStudio

3D Digital PCR Chip Case Lid

• QuantStudio

3D Digital PCR Sample Loading Blade

Open the QuantStudio

3D Digital PCR 20K Chip package, gently grasp the chip

by its sides, then place it face-up on the heated block, preheated to 40°C.

IMPORTANT! Allow the

Digital PCR 20K Chip to sit on the heated block for at

least 5 seconds before loading it.

40±1°C

Load the PCR reaction onto the Digital PCR 20K Chip:

Briefly vortex and centrifuge the prepared reaction (master mix, assay, and

template), then aspirate 14.5 μL of the solution into a P20 pipette. If the

reactions were placed on ice, allow them to warm prior to loading.

While holding the Sample Loading Blade

by its edges, carefully dispense the

reaction into the sample-loading port. After loading, gently tap the top of

the Sample Loading Blade to spread the reaction across the full length of the

blade.

While filling the Sample Loading Blade:

• Hold the pipette so that the tip meets the sample-loading port at a

sharp angle and so it does not deflect either blade (forward or rear).

• Do not depress the pipette to the second stop. Doing so increases the

chance of introducing an air bubble to the Sample Loading Blade.

• Avoid creating air bubbles.

Chapter 2 Prepare Samples and Load Reactions

Load chips manually

QuantStudio

3D Digital PCR System User Guide