User guide

Table Of Contents

• Immersion Fluid Tip

•

UV-Activated Chip Sealant Syringe

• UV-Curing Stylus for Chip Sealant

• UV-Curing Stylus Stand

• Isopropanol

• Gloves, powder-free, nitrile

• Heated block (capable of maintaining 40±1°C)

• Scissors

• Cleanroom-grade, low-lint polyester wipes

• Microcentrifuge

• Pipettes and tips, P10 to P1000

• Vortexer

WARNING! ULTRAVIOLET LIGHT HAZARD. Looking directly at a UV light

source can cause serious eye damage. Never look directly at a UV light source

and always prevent others from UV exposure. Follow the manufacturer’s

recommendations for appropriate protective eyewear and clothing.

IMPORTANT! Wear powder-free gloves while preparing the QuantStudio

Digital PCR 20K Chips.

Prepare the workspace:

Preheat the heated block to 40°C, the optimal temperature for loading

Digital PCR 20K Chips.

Note: The loading surface should be a flat, temperature-controlled surface

that is large enough to accommodate the Digital PCR 20K Chip consumable.

Allow the prepared digital PCR reaction to equilibrate to room temperature

(approximately 15 minutes).

If necessary, replace the batteries of the UV Stylus in the

UV Stylus Stand.

Note: Replace the batteries of the UV Stylus after 10 hours of use.

Prepare the syringe containing Chip Sealant:

Remove the syringe, plunger, and tip from the protective packaging.

IMPORTANT! Do not discard the brown plastic bag provided. When not in

use, store the Chip Sealant syringe within the bag and in a dark location.

Remove the protective caps from both ends of the syringe, push and twist

the tip to lock it into place, then insert the plunger into the opposite end of

the syringe.

Place the assembled syringe within its package until ready for use.

Load and seal the

Digital PCR 20K

Chips

Chapter 2 Prepare Samples and Load Reactions

Load chips manually

QuantStudio

3D Digital PCR System User Guide