User guide

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CHAPTER 4 Analyze the Prepared Chips ............................. 66
Using the QuantStudio
®
3D Digital PCR Instrument ................................. 66
About imaging and primary analysis ......................................... 66
Analysis workflow ......................................................... 67
About the instrument interface .............................................. 68
Analyze the chips .............................................................. 69
Materials required ......................................................... 69
Guidelines for high-throughput analysis ...................................... 69
Prepare the instrument for imaging data ..................................... 69
Image the Digital PCR 20K Chips ............................................. 70
Analyzing the data ............................................................. 73
About the analysis ......................................................... 73
About the results screen ................................................... 74
About data quality flags ..................................................... 75
About the data files ........................................................ 75
Transfer the results ........................................................ 76
Using the QuantStudio
®
3D AnalysisSuite
™
Software ............................... 77
Access the software from a cloud account .................................... 78
Obtain information from the Help system ..................................... 78
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CHAPTER 5 Troubleshooting .......................................... 79
Troubleshooting chip images using the Chip View .................................. 79
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APPENDIX A Maintenance ............................................. 86
Cleaning the chip tray and sample block .......................................... 86
Materials required ......................................................... 86
Clean the chip tray or sample block .......................................... 87
Configure the instrument settings ............................................... 88
(Optional) Set the instrument name .......................................... 88
Set the date and time ....................................................... 89
Returning your QuantStudio
®
3D Instrument for service ............................ 89
Replace the instrument fuses ................................................... 90
Materials required ......................................................... 90
Replace the fuses .......................................................... 91
Update the instrument firmware ................................................. 91
Update the firmware ....................................................... 92
Calibrate the touchscreen ....................................................... 93
View the instrument log ......................................................... 93
View the log ............................................................... 93
Contents
QuantStudio
®
3D Digital PCR System User Guide 5