User guide

Table Of Contents

Seal the Chip Case using Chip Sealant:

Hold the syringe tip just above (or in slight contact with the inside wall) of

the fill port of the sealed Chip Case, then carefully fill the port with Chip

Sealant, ensuring that the fluid touches the walls of the port. To ensure the

seal, create a dome of sealant over the top of the port.

IMPORTANT! When applying the Chip Sealant:

Apply the Chip Sealant only to the fill port. Do not apply the sealant to

the Chip ID or Chip Case Lid window.

When not in use, store the Chip Sealant with the syringe tip attached tip-

first inside the brown plastic bag provided to prevent the sealant in the

syringe from curing.

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Insert the Digital PCR 20K Chip

assembly into the UV-Curing Station on the

Chip Loader. Push the chip into the station until the ultraviolet light

illuminates.

IMPORTANT! Make sure that the chip is oriented so that the fill port is

directly beneath the beam of ultraviolet light.

IMPORTANT! Do not allow the Digital PCR 20K Chip to contact the roof of

the UV-Curing Station.

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>15 sec

Chapter 2 Prepare Samples and Load Reactions

Load the chips using the Chip Loader

QuantStudio

3D Digital PCR System User Guide