User guide

Table Of Contents

Rotate the Chip Loader arm so that the Chip Case Lid solidly contacts the Digital

PCR 20K Chip, firmly press down for 15 seconds to ensure a tight seal, then press

the Lid Nest button and return the Chip Loader arm to its original position.

>15 sec

IMPORTANT! While applying the lid:

Press down on the Chip Loader until you reach a hard stop. The arm requires

>20 lbs of force to apply the Chip Case Lid. If you suspect that you are

applying insufficient pressure, increase the duration of the press (>15 seconds).

Press the Lid Nest button before you retract the Chip Loader arm.

Release the Chip Loader arm gently and do not press down again to avoid

creating a vacuum.

Fill the Chip Case with Immersion Fluid:

Hold the Chip Case by its edges and at a 45° angle so that air can escape

from the loading port as you fill it, then carefully dispense Immersion Fluid

into the port until the

Chip Case contains an air bubble no larger than the fill

port (<2-3 mm in diameter).

After filling the Immersion Fluid, rotate it slightly to reveal any air bubbles

that might be hidden behind the serial number label. If bubbles are present,

add additional Immersion Fluid to remove them.

Note: Minimize air bubble creation when you dispense the fluid; however,

one small air bubble (<2-3 mm in diameter) is acceptable.

X0300036

Using a low-lint wipe, remove any excess

Immersion Fluid from the Chip

Case to ensure optimal imaging on the QuantStudio

3D Instrument.

Chapter 2 Prepare Samples and Load Reactions

Load the chips using the Chip Loader

QuantStudio

3D Digital PCR System User Guide