User guide

e.
Press the black loading button on the Chip Loader to load the Digital PCR
20K Chip.
IMPORTANT! Before pressing the loading button, confirm that the Sample
Loading Blade is firmly seated on the loader arm.
The status light flashes green during the loading sequence, and displays
solid green when finished.
f.
After the Chip Loader loads the Digital PCR 20K Chip, immediately add
several drops of
Immersion Fluid directly onto the chip so that the fluid
covers the entire surface of the chip. If any fluid is present on the edges of
the case that will contact the lid, remove it with a low-lint wipe that has
been sprayed with isopropanol.
IMPORTANT! When applying the
Immersion Fluid:
·
Expel the Immersion Fluid slowly. If injected too quickly, the fluid can
flush the reactions suspended within the chip.
·
Cover the entire surface of the chip. Failure to do so will lead to
evaporation of the reactions in the exposed wells.
·
Pipet the Immersion Fluid only onto the chip. A small amount of fluid
running off the surface of the chip is acceptable. Do not fill the case with
fluid.
·
Do not allow the syringe tip to contact the Digital PCR 20K Chip.
Correct
Fluid on
edges
Incorrect
Insufficient
fluid
Fluid over
chip
Chapter 2 Prepare Samples and Load Reactions
Load the chips using the Chip Loader
2
QuantStudio
®
3D Digital PCR System User Guide 47