User guide

Table Of Contents

Remove the red protective film from a QuantStudio

3D Digital PCR Chip

Case Lid, press the Lid Nest button ( ), carefully place the Chip Case Lid

into the Lid Nest as shown ( ), then release the button to clamp the chip in

place.

IMPORTANT! Before applying the Chip Case Lid, make certain that it is

correctly oriented to the Digital PCR 20K Chip assembly.

Briefly vortex and centrifuge the prepared digital PCR reaction (master mix,

assay, and template), then carefully transfer 14.5 μL of the solution into the

sample-loading port of the Sample Loading Blade

. If the reactions were

placed on ice, allow them to warm prior to loading.

While filling the Sample Loading Blade:

• Hold the pipette so that the tip meets the sample-loading port at a

sharp angle and so it does not deflect either blade (forward or rear).

• Do not depress the pipette to the second stop. Doing so increases the

chance of introducing an air bubble to the Sample Loading Blade.

• Avoid creating air bubbles.

Note: Although the master mix may not distribute evenly in the Sample

Loading Blade immediately after loading, the fluid will spread across the

blade prior to loading.

Chapter 2 Prepare Samples and Load Reactions

Load the chips using the Chip Loader

QuantStudio

3D Digital PCR System User Guide