User guide

4.
Load the PCR reaction onto a Digital PCR 20K Chip:
a.
Open the QuantStudio
®
3D Digital PCR 20K Chip package, then gently
grasp the chip by its sides and load it face-up into the chip nest. Lock the
Digital PCR 20K Chip into place by pressing down the chip nest lever ( )
prior to placing the chip into the chip nest as shown ( ).
IMPORTANT! Use the image next to the chip nest to correctly orient the
Digital PCR 20K Chip within the Chip Loader.
2
1
b.
Press the sample loading blade lever ( ), then install the QuantStudio
®
3D
Digital PCR Sample Loading Blade to the Chip Loader as shown below.
IMPORTANT! Press the
Sample Loading Blade against the loader head (
)
to confirm that the blade is properly seated.
2
1
Chapter 2 Prepare Samples and Load Reactions
Load the chips using the Chip Loader
2
QuantStudio
®
3D Digital PCR System User Guide 45