User guide

Recovering from a Chip Loader error
Perform the following steps if the QuantStudio
®
3D Digital PCR Chip Loader status
light flashes red, indicating that it has encountered an error.
1.
Power off the Chip Loader, wait 30 seconds, then power it on.
2.
If the status light is still flashing red, press the load button and record the
sequence of colors displayed.
3.
Contact technical support (see “Customer and technical support“ on page 121).
WARNING! ULTRAVIOLET LIGHT HAZARD. Looking directly at a UV light
source can cause serious eye damage. Never look directly at a UV light source
and always prevent others from UV exposure. Wear appropriate protective
eyewear and clothing.
IMPORTANT! Wear powder-free gloves while preparing QuantStudio
®
3D Digital
PCR 20K Chips.
IMPORTANT! The QuantStudio
®
3D Digital PCR Chip Loader must be placed on a
level surface to ensure consistent loading across the
Digital PCR 20K Chips.
1.
Prepare the workspace:
a.
Plug in and power on the QuantStudio
®
3D Digital PCR Chip Loader, then
wait until the
Chip Loader status light illuminates solid green, indicating
that the chip nest has reached operating temperature (£20 minutes
depending on room temperature).
b.
Allow the prepared digital PCR reaction to equilibrate to room temperature
(approximately 15 minutes).
c.
Remove the following consumables from their packaging and place them on
a clean, dry, lint-free surface:
• QuantStudio
®
3D Digital PCR Chip Case Lid
• QuantStudio
®
3D Digital PCR Sample Loading Blade
2.
Prepare the syringe containing Chip Sealant:
a.
Remove the syringe, plunger, and tip from the protective packaging.
IMPORTANT! Do not discard the brown plastic bag provided. When not in
use, store the Chip Sealant syringe within the bag and in a dark location.
b.
Remove the protective caps from both ends of the syringe, twist and push
the tip to lock it into place, then insert the plunger into the opposite end of
the syringe.
c.
Place the assembled syringe within its protective package until ready for
use.
Load and seal the
chips using a Chip
Loader
Chapter 2 Prepare Samples and Load Reactions
Load the chips using the Chip Loader
2
QuantStudio
®
3D Digital PCR System User Guide 43