User guide

Load the chips using the Chip Loader
The following procedure explains how to load QuantStudio
®
3D Digital PCR 20K
Chips automatically using a QuantStudio
®
3D Digital PCR Chip Loader. This
procedure assumes that you have prepared your PCR reactions as explained in
“Prepare the reaction mix and samples“ on page 39 and are ready to load them onto
Digital PCR 20K Chips.
The following materials are required to load and seal QuantStudio
®
3D Digital PCR
20K Chips for use on the QuantStudio
®
3D Digital PCR System.
• Prepared digital PCR reactions (from the previous section)
• QuantStudio
®
3D Digital PCR 20K Chips
• QuantStudio
®
3D Digital PCR Chip Case Lids
• QuantStudio
®
3D Digital PCR Sample Loading Blades
• Immersion Fluid
• Immersion Fluid Tip
• QuantStudio
®
3D Digital PCR Chip Loader
• UV-Activated Chip Sealant Syringe
• Isopropanol
• Gloves, powder-free, nitrile
• Scissors
• Cleanroom-grade, low-lint polyester wipes
• Microcentrifuge
• Pipettes and tips, P10 to P1000
• Vortexer
The LED on top of the QuantStudio
®
3D Digital PCR Chip Loader indicates the
instrument status. The light is illuminated at all times when the Chip Loader is
powered on and changes color according to instrument readiness.
Color State Status
Blue Solid The Chip Loader is booting.
Flashing The chip nest is not at temperature or the lever arm is not fully
open.
Green Solid The Chip Loader is at the correct temperature and ready to load a
chip.
Flashing The Chip Loader is loading a Digital PCR 20K Chip.
Red Flashing The Chip Loader has encountered an error.
Materials
required
QuantStudio
®
3D
Digital PCR Chip
Loader status
light
Chapter 2 Prepare Samples and Load Reactions
Load the chips using the Chip Loader
2
42 QuantStudio
®
3D Digital PCR System User Guide