User guide

To ensure the proper processing and analysis of your loaded Digital PCR 20K Chip,
handle them according to the following guidelines:
•
Always wear powder-free gloves when loading and sealing Digital PCR 20K
Chips.
IMPORTANT! Never handle Digital PCR 20K Chips or Chip Case Lids without
gloves. Oils from your hands can contaminate the components and interfere with
thermal cycling and imaging.
• Handle the Digital PCR 20K Chips and Chip Case Lids as follows:
– Hold the Digital PCR 20K Chips and Chip Case Lids by gently gripping
them by their sides.
– Do not touch the surface of the Digital PCR 20K Chips. If you accidentally
touch the surface of a chip, discard it.
Note: Debris or excess sample on the Chip Case Lid can fluoresce and
interfere with thermal uniformity.
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• If necessary, use a permanent pen or marker to label the back of each loaded Chip
Case to aid in sample tracking.
•
Load each Digital PCR 20K Chip within 2 hours after opening it.
• Load Digital PCR 20K Chips in alphanumeric order (according to Digital PCR
20K Chip serial number) to avoid data-entry errors.
• Apply a Chip Case Lid and add Immersion Fluid to each Digital PCR 20K Chip
immediately after loading it to avoid evaporation.
• Load and seal Digital PCR 20K Chips in batches of up to 24 chips (the maximum
number that can be loaded onto one thermal cycler).
• If you do not intend to load an opened Digital PCR 20K Chip immediately, cover
the chip using the aluminum plate found within the packaging to prevent
contamination.
• When not in use, store the Chip Sealant within its original protective package to
prevent the sealant in the syringe tip from curing. The Chip Sealant can be stored
with the syringe tip attached.
• Use all of the Immersion Fluid within 60 minutes of uncapping the syringe. Once
a syringe is opened, you cannot reattach the cap for later use.
• Thermal cycle Digital PCR 20K Chips within 2 hours after loading them.
Guidelines for
loading and
sealing Digital
PCR 20K Chips
Chapter 2 Prepare Samples and Load Reactions
Load the Digital PCR 20K Chips
2
QuantStudio
®
3D Digital PCR System User Guide 41